The Two-Component System ArlRS and Alterations in Metabolism Enable Staphylococcus aureus to Resist Calprotectin-Induced Manganese Starvation
Fig 4
ArlRS is necessary for establishing invasive S. aureus infection and for resisting Mn sequestration during infection.
Wild type C57BL/6 (C57) and CP-deficient C57BL/6 S100A9-/- (CP-/-) mice were infected with either S. aureus Newman (wild type) or ΔarlRS and (A) mean weight loss and (B-C) bacterial burdens in the (B) liver and (C) heart and kidneys were assessed after four days of infection. (A) See S3 Fig for pair wise comparisons that include error bars. * = p≤ 0.05 relative to C57BL/6 mice infected with wild type S. aureus and # = p≤ 0.05 relative to C57BL/6 mice infected with ΔarlRS by two-way ANOVA with Tukey’s posttest corrected for repeated measurements. (B-C) * = p≤0.05 as determined by Mann-Whitney test. The lines indicate the mean. The data are the results from three independent experiments. See also S3 Fig.