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Variant Exported Blood-Stage Proteins Encoded by Plasmodium Multigene Families Are Expressed in Liver Stages Where They Are Exported into the Parasitophorous Vacuole

Fig 1

Maximum likelihood phylogeny of fam-a gene sequences from Plasmodium spp.

The tree was estimated using RAxML and a GTR+Γ model. Branches subtended by nodes with >75 bootstrap support are shown in bold. Robust basal nodes are indicated by black squares with bootstrap proportions (above node) and Bayesian posterior probabilities (beneath node). At right, coloured blocks indicate the species to which a terminal node belongs. Clades of orthologs that display positional conservation are indicated with green blocks; where a sequence has been lost secondarily in a species is shown by an ‘X’. The tree is rooted using an out-group comprising single copy fam-a orthologs from primate Plasmodium species. The phylogeny is subdivided into four sections: genes located at the conserved, ‘ancestral’ locus on chromosome 13 (below line i); genes found at loci conserved across RMP species (between lines i and ii); and a robust clade of species-specific paralogs derived from a conserved locus on chromosome 6 or 13 (between lines ii and iii); a robust clade of species-specific paralogs derived from a conserved locus on chromosome 8 (above line iii).Transcription levels (shown as different coloured and sized circles) in blood stages are shown for individual genes based on RNAseq data (FPKM values) (from [33] and S1 Table). Expression levels as shown by four different sized circles: Class 1 (smallest circle): 2-8x the threshold level; class 2: 8-16x the threshold; class 3 (largest circle): >16x the threshold.

Fig 1

doi: https://doi.org/10.1371/journal.ppat.1005917.g001