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Inactivation of Prions and Amyloid Seeds with Hypochlorous Acid

Fig 4

Inactivation of steel-bound prion seeding activity.

A. RT-QuIC reaction wells were seeded with a 3–4 mm segment of stainless steel wire pre-coated with hamster scrapie (red) or normal BH (gray) at tissue dilutions of 10−3–10−10 as indicated. B. Wire segments pre-coated with ScBH at a 10−3 tissue dilution were submersed for 1 h in saline (mock disinfectant; red), BrioHOCl (blue), 40% bleach (2.4% hypochlorite; green), 1 N NaOH (black) or 2% Environ LpH (purple) as indicated prior to RT-QuIC analysis using hamster (90–231) rPrPC substrate. Each trace represents the average normalized ThT fluorescence of 4 replicate wells.

Fig 4

doi: https://doi.org/10.1371/journal.ppat.1005914.g004