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Inactivation of Prions and Amyloid Seeds with Hypochlorous Acid

Fig 2

Inactivation of sCJD (A), vCJD (B), BSE (C), CWD (D) & sheep scrapie (E) seeding activity in brain homogenates by BrioHOCl.

The indicated BH samples (10%) were pretreated for 1 h with 100 volumes of BrioHOCl (blue), or saline (red) as a mock treatment control. Similar HOCl and saline pretreatments of NBH are indicated in gray. Resulting samples were then subjected to serial 10-fold dilutions and RT-QuIC analysis was performed using 10−4 through 10−11 tissue dilutions as indicated. Hamster (90–231) rPrPC was used as substrate for the sCJD and CWD reactions while chimeric hamster-sheep rPrPC was used as substrate for the vCJD, BSE and sheep scrapie reactions. Each trace represents the average normalized ThT fluorescence of 4 replicate wells.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1005914.g002