Leader-Containing Uncapped Viral Transcript Activates RIG-I in Antiviral Stress Granules
Fig 3
RIG-I localized in NDV vRC and avSG.
(A and B) HeLa cells were either mock treated or infected with NDV (MOI = 1) for 6 and 12 hours. Cells were immunostained for RIG-I (green), N (red), and TIAR (white) (A), or RIG-I (green), TIAR (red), and IRF-3 (white) (B). (C) FLAG-IPS-1/HeLa cells were either mock treated or infected with NDV (MOI = 1) for 6 and 12 hours. The cells were immunostained for FLAG (green), L (red), and TIAR (white). Nuclei were stained with DAPI (blue). The white scale bar corresponds to 10 μm. The boxed area was enlarged and displayed on the right (zoom). Partial co-localization between IPS-1 and L (vRC) or TIAR (avSG) is shown by displaying them in green and red, respectively (zoom).