Blimp-1-Dependent IL-10 Production by Tr1 Cells Regulates TNF-Mediated Tissue Pathology
Fig 2
Blimp-1 controls CD4+ T cell derived IL-10 production.
(A) Prdm1GFP/+ (black-filled histograms) and WT (grey-filled histograms) mice were infected with PcAS. Blimp-1/GFP expression measured on CD4+ T cells producing IL-10, IFNγ and TNF at day 15 p.i.. (B) Kinetics of Blimp-1/GFP expression measured in various cytokine producing CD4+ T cells, as indicated, beginning at day 5 p.i.. (C) Prdm1GFP/+ and WT mice were infected with L. donovani and the kinetics of Blimp-1 expression measured in the spleen and liver. (D) FACS plots showing gated CD4+ T cell IL-10 and IFNγ production from Prdm1GFP/+ mice treated with 500 μg of polyclonal rat IgG (black bars in (E)) or anti-IL-12 (C17.8; white bars in (E)), as indicated, prior to infection and every 3 days following infection until day 14 p.i.. (E) The frequencies of IL-10 and IFNγ-producing CD4+ and CD8+ T cells, as well as Blimp-1 expressing CD4+ and CD8+ T cells, were determined by flow cytometry at day 14 p.i.. Representative of 3 similar experiments, mean ±SEM, n = 5 in each group in each experiment, **p<0.01, Mann-Whitney U test.