Interferon-γ Inhibits Ebola Virus Infection
Fig 3
Human MDM gene expression is altered by 24 hours of IFNγ treatment.
M-CSF cultured hMDMs were incubated with or without IFNγ for 24 hours and RNA was harvested for gene arrays. (A) Heat map summaries of hMDM gene expression significantly altered by IFNγ. Human MDMs were obtained from four different volunteers and treated with M-CSF or M-CSF plus IFNγ. Genes were clustered into annotated ontology groups and listed in the corresponding GO groups table with their respective GO terms. Significance was determined by paired t-test analysis with cutoff values of at least two-fold change and p < 0.01. NUSE analysis of the array demonstrated that the means were centered at a value of 1 and minimum and maximum values between 0.95 and 1.05. ISGs that were assessed further in this study are bolded in the GO table. (B) Venn diagram representing the statistically significant individual and shared genes altered by IFNγ in hMDMs and human alveolar macrophages.