Modulation of the Surface Proteome through Multiple Ubiquitylation Pathways in African Trypanosomes
Fig 1
The effect of AP-1γ RNAi on markers of the endocytic pathway.
All experiments were performed in uninduced (-) or induced (+) cells for 18 hours. (A) Western blot and (B) immunofluorescence for GLP-1, CatL, MFST12myc, (C) ISG65 and ISG75. In B and C scale bar = 2.5 μm, protein antigens are shown in red, DNA visualised with DAPI is in blue. (D) AP-1γ RNAi cells were subjected to RNAi knockdown followed by cycloheximide treatment. Cells were harvested at various time points and endogenous ISG65 and ISG75 levels were monitored by western immunoblotting. β-tubulin was used as a loading control. Lower panel shows quantification, with open symbols induced (+) and closed symbols uninduced (-) and representing the mean of three independent experiments, with the standard error indicated.