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Heterosexual Transmission of Subtype C HIV-1 Selects Consensus-Like Variants without Increased Replicative Capacity or Interferon-α Resistance

Fig 5

In vitro replication of TF and NT viruses in PBMC.

(A) Virus growth over 10 days in PBMC culture as measured by reverse transcriptase (RT) activity (DLU = digital light units) of the TF (blue), NT variants (red) and MJ4 standard (black) for one representative transmission pair, 331. (B) Replicative capacity (RC) scores, based on the area under the curve relative to MJ4, of all tested TF (blue) and NT (red) variants from six transmission pairs. (C) RC scores of the TF (blue) compared to the median of the corresponding NT variants (red) (Wilcoxon matched-pairs signed rank test, two-tailed p = 0.219). (D) Spearman correlation of the pairwise distance to the amino acid subtype C consensus described in Fig 2 and RC scores over all variants tested (Non-parametric Spearman p = 0.0158, r = 0.4168.) The linear regression line is shown for visualization purposes.

Fig 5