Adipose Tissue Is a Neglected Viral Reservoir and an Inflammatory Site during Chronic HIV and SIV Infection
Fig 5
The influence of SIV infection on adipose tissue macrophage numbers and phenotypes.
(A) The percentage of CD14-expressing cells among CD45+ cells recovered in the SVF from SCAT and VAT from non-infected animals (open circles, n = 13) and SIV-infected animals (filled squares, n = 9). (B) Representative adipose tissue sections, confirming the presence of macrophages in adipose tissue in immunochemical preparations (CD68 staining). Scale bar: 50 μm, magnification x400. (C, D) Analyses of CD206 and CD163 expression on adipose-resident CD14-expressing cells recovered from SCAT (C) and PBMCs (D) from non-infected animals (open circles, n = 11) and SIV-infected animals (filled squares, n = 9). CD206-expressing fractions were not detected in PBMCs. Gating strategies are shown in S4 Fig. Data are quoted as the median [interquartile range]. Significant differences in a Mann-Whitney non-parametric test are shown as * p<0.05.