Intracellular Survival of Leishmania major Depends on Uptake and Degradation of Extracellular Matrix Glycosaminoglycans by Macrophages
Fig 2
The L. major ∆gnat mutant is a GlcNAc auxotroph.
(A) Wild type (WT) and ∆gnat promastigotes were cultivated in M199 medium supplemented with 50 μg/ml GlcN or GlcNAc and parasite growth monitored by measuring optical density at 600nm. (B) ∆gnat growth in the absence of GlcNAc was rescued by ectopic expression of GNAT from pXG-PURO plasmid, ∆gnat [pX-PURO-GNAT], in M199. ∆gnat mutant was incubated in (C) limiting or (D) increasing concentrations of GlcNAc and growth monitored over time. Data represents mean from three biological repeats.