Crystal Structure of USP7 Ubiquitin-like Domains with an ICP0 Peptide Reveals a Novel Mechanism Used by Viral and Cellular Proteins to Target USP7
Fig 5
The D762R/D764R (MRGR) mutation disrupts ICP0, GMPS and UHRF1 binding to USP7 in human cells.
(A) 293T cells were transfected with plasmid expressing myc-tagged WT or D762R/D764R (MRGR) USP7 and ICP0 or with WT USP7 or ICP0 expression plasmids alone. USP7 was precipitated with anti-myc antibody and recovered proteins were detected by Western blotting with antibodies against myc and ICP0. (B) 293T cells were transfected with plasmid expressing myc-tagged WT or D762R/D764R (MRGR) USP7 or with empty plasmid (None). USP7 was precipitated with anti-myc antibody and recovered proteins were detected by Western blotting with antibodies against myc, GMPS or UHRF1.