Two Cdc2 Kinase Genes with Distinct Functions in Vegetative and Infectious Hyphae in Fusarium graminearum
Fig 1
Two Cdc2 co-orthologs in F. graminearum.
(A) Tertiary structures of Cdc2A and Cdc2B predicted by I-TASSER server (http://zhanglab.ccmb.med.umich.edu/I-TASSER/). The N- and C-terminals, the key landmarks including the PSTAIRE helix and the T-loop (activation loop), and the inhibitory (Tyr15) and activating (Thr181) phosphorylation residues [1, 4] are indicated. Yellow and green colors indicate amino acid sites with type I and type II functional divergence between Cdc2A and Cdc2B, respectively. The high sequence variation region between Cdc2A and Cdc2B was also indicated. For detailed residues please see S1 Fig. (B) Colony morphology of the wild type (PH-1), cdc2A and cdc2B deletion mutants (C2A1 and C2B1), and the complemented transformants (C2A-N4 and C2B-N2). (C) Growth rate and conidiation of PH-1, C2A1, C2B1, C2A-N4, and C2B-N2. Growth rate was measured with race tube cultures (PDA) after incubating at 25°C for 10 days. Conidiation was assayed with 5-day-old CMC cultures. Mean and standard deviation were calculated from data of three independent experiments.