CD200 Receptor Restriction of Myeloid Cell Responses Antagonizes Antiviral Immunity and Facilitates Cytomegalovirus Persistence within Mucosal Tissue
Figure 1
CD200R promotes MCMV persistence in the mucosa.
(A-C) MCMV titres in the spleen (A), liver (B) and SGs (C) were measured 4 and 7 (A&B) or 7, 14 and 33 (C) days pi. Individual mice and median are shown. Horizontal dashed lines depict the lower limit of detection. (D) Histogram overlays of CD200R expression by SG leukocytes at day 7 pi. Blue line = wt, shaded = CD200R-/-. (E&F) Intensity of surface CD200R expression by APCs (E) isolated from the SGs and spleens of wt mice is represented as median fluorescent intensity (MFI). Results represent the mean +/- SEM of 3 mice. (F) Wt and IL-10-/- mice were infected with MCMV and on 7 or 14 days pi CD200R expression by SG-APCs was analyzed by flow cytometry. Fluorescence Minus One (FMO) control = SG-APCs from infected wt mice 7 (left) or 14 (right) days pi. Plots are representative of 3–4 mice. All data is representative of 2–3 separate experiments.