Advertisement

< Back to Article

IL-28B is a Key Regulator of B- and T-Cell Vaccine Responses against Influenza

Figure 3

The minor-allele IL-28B genotype is associated with significantly lower expression of IL-28B, but not IL-29 or IL-28A mRNA in healthy volunteers.

(A) PBMCs from healthy volunteers were stimulated for six-hours with H1N1. mRNA-expression of IL-28A, IL-28B and IL-29 were normalized to non-stimulated controls and HPRT expression. Major (rs8099917 TT, n = 28) compared to minor (rs8099917 non-TT, n = 21) allele genotype. MWU-test determined statistically significant differences between groups. Bars show median values with interquartile range. (B) PBMCs from healthy volunteers (n = 25) were pre-treated with mycophenolate mofetil for 2 h prior to stimulated for six-hours with H1N1. mRNA-expression of IL-28A, IL-28B and IL-29 and statistical analysis were performed using the Kruskal Wallis (KW) test.

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1004556.g003