Crystal Structure of Calcium Binding Protein-5 from Entamoeba histolytica and Its Involvement in Initiation of Phagocytosis of Human Erythrocytes
Figure 4
Interaction of EhCaBP5 with myosin 1B.
Total (800 µg) E. histolytica lysate was incubated with Sepharose-anti-CaBP5 antibody conjugate for 6 h at 4°C with shaking. The beads were then washed and the bound material was then eluted and analysed by western blotting followed by immunostained with anti-myosin 1B antibody raised in rabbit. The blot was reprobed with anti-CaBP5 antibody raised in mice. The total input lysate was also probed for the presence of EhCaBP5 and myosin 1B by their respective antibodies.