GAPDH-A Recruits a Plant Virus Movement Protein to Cortical Virus Replication Complexes to Facilitate Viral Cell-to-Cell Movement
Figure 5
NbGAPDH-A changes its subcellular localization in association with RCNMV RNA replication, but not with viral proteins.
Representative CLSM images of agroinfiltrated N. benthamiana epidermal cells. Each Agrobacterium culture was diluted to OD600 = 0.8 and equal volume of cultures were mixed for infiltration. Confocal microscopy images were taken at 40 h after infiltration. Images present confocal projections composed of 15 optical sections taken at 1 µm intervals, from the surface to the middle of epidermal cells. Chloroplast autofluorescence was detected in all the RFP-channel images by the use of 610IF emission filter. Scale bar = 20 µm. (A) Epidermal cells transiently expressing NbGAPDH-A-GFP (left two panels), and NbGAPDH-A-GFP with MP-mCherry (right four panels). Arrows represent PD-localized MP-mCherry signals. (B) Epidermal cells, which transiently expressed NbGAPDH-A-GFP (panels 1 and 2), or NbGAPDH-A-GFP with RCNMV RNA1 and ER marker (ER-mCherry) (panels 3 and 4), or NbGAPDH-A-GFP with viral replicase component proteins p27 and p88 (panels 5 and 6). Arrowheads represent chloroplast-localized NbGAPDH-A-GFP signals and arrows represent cortical ER-localized NbGAPDH-A-GFP signals.