Glutamate Secretion and Metabotropic Glutamate Receptor 1 Expression during Kaposi's Sarcoma-Associated Herpesvirus Infection Promotes Cell Proliferation
Figure 7
KSHV latency associated Kaposin A protein mediates mGluR1 expression.
A) Kaposin A (ORFK12) over expression leads to mGluR1 expression in BJAB cells. BJAB cells were transduced with control lentivirus vector or lentiviruses expressing ORFs 71, -72, -73 and Kaposin A for 72 h. The expression levels of mGluR1 in the cell lysates were determined by Western blot using anti-mGluR1 antibody. B) mGluR1 and Kaposin A expression determined by Western blot in primary HMVEC-d cells following transduction with lentivirus control or lentivirus-Kaposin A. β-actin was used as loading control. C) Colocalization of REST and Kaposin A in HMVEC-d cells transduced with lentivirus vector, or lentivirus-Kaposin A after 72 h. Boxed areas are enlarged in the right panel. Arrows indicate colocalization of REST and Kaposin A. Magnifications 40×.