Myeloid Derived Hypoxia Inducible Factor 1-alpha Is Required for Protection against Pulmonary Aspergillus fumigatus Infection
Figure 2
Myeloid HIF1α is required for fungal clearance and survival following A. fumigatus pulmonary challenge.
Immune competent littermate (Cre-/HIF1α floxed: WT) and HIFC mice received 7×107 conidia i.t. and were A) monitored for survival (Log-Rank Test, # = p<0.0001, repeated twice) and B) Fungal burden on days 1 and 2 post challenge. Data represent 5 biological and 3 technical replicates for each time point. Representative histology of H&E or GMS stained lung sections from C) HIFC and D) WT mice at 12 and 24 hr post challenge. Left image (5×), right image (20×). Levels of E) Albumin and F) LDH were measured in the BALFs at 4, 8, 12, and 24 (LDH) hrs of WT and HIFC mice challenged with 7×107 conidia (Af) or PBS (4–8 biological replicates for each time point). * indicates P value of <0.02, ** indicates P value of <0.05 (unpaired Students t test).