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Dynamics of HIV Latency and Reactivation in a Primary CD4+ T Cell Model

Figure 1

Viral production from latently infected cells ex vivo.

Panel A. HIV RNA copies/ml measured in the supernatant of resting CD4+ T cells isolated from treated HIV+ individuals (S: cells from single individuals, P: pooled cells from 2-3 individuals) and stimulated ex vivo with DMSO (control, black), SAHA (blue) or TCR (green) for 1, 2 or 4 days. Panel B. Box plot showing HIV RNA copies/ml measured in the supernatant of resting CD4+ T cells isolated from treated HIV+ individuals and stimulated ex vivo with DMSO (control, black), SAHA (blue) or TCR (green) for 1, 2 or 4 days. Each circle represents a different cellular sample. Bars: min to max. Solid line: median. Panel C. Immunoblot of histones (1 µg) extracted from stimulated ex vivo cells isolated from treated HIV+ individuals. H3K27Ac: anti-histone 3, acetylated Lysine 27; H3K9Ac: anti-histone 3, acetylated Lysine 9; H3: anti-histone 3 (total).

Figure 1

doi: https://doi.org/10.1371/journal.ppat.1004156.g001