Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation
a) Typical example after 1 h incubation with Cy3-DNA and subsequent washing. Left: superposition of brightfield and fluorescence image, right: fluorescence image. b) Typical example of the same sample after washing and subsequent incubation with unlabeled DNA for another 1 h. left: superposition of brightfield and fluorescence image, right: fluorescence image. c) Fraction of cells with foci at septum directly after 60 min incubation with Cy3-DNA, after 60 min incubation with medium, and after 60 min incubation with unlabeled DNA. black: ΔpilV, grey: ΔcomA ΔpilV. (N>1000 for each condition) d) Time-lapse of central focus formation and movement. Upper line: brightfield image, middle line: fluorescence image, lower line: pseudocolored intensity image. The arrow denotes the timepoint where the focus moves. Δt = 6 min.