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Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation

Figure 10

Spatial distribution of Cy3-DNA foci after 15 min of exposure to transforming Cy3-DNA in ΔpilV and wt.

a) Typical examples of Cy3-DNA single cell fluorescence for different fragment lengths (ΔpilV). The white line denotes the cell contour as obtained from the bright field image. b) Two-dimensional distribution of focus location (ΔpilV). The color encodes the probability for finding a focus within the 2D bin. (N>18000 for each condition). c) Two-dimensional distribution 10% brightest foci (ΔpilV). d, e) Typical examples of Cy3-DNA single cell fluorescence for different fragment lengths (wt). f) Two-dimensional distribution of focus location (wt) (N = 309).

Figure 10

doi: https://doi.org/10.1371/journal.ppat.1004043.g010