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Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation

Figure 8

Dynamics of focus formation (ΔpilV) with 10 kbp fragments of Cy3-DNA.

a, c) Examples for time lapse of binding and import. Upper line: brightfield image, middle line: fluorescence image, lower line: pseudocolored intensity image. Δt = 3 min b, d) Fluorescence intensities of cells shown in a, c). e) Distribution of initial location of Cy3-DNA foci normalized to length of diplococcus. Black circles: Cy3-DNA, red squares: YOYO-DNA.

Figure 8