Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation
a, b) Probability distribution of the total fluorescence intensity of individual a) ΔpilV and b) ΔcomE34 ΔpilV as a function of time. c, d) Typical fluorescence images after 1 h for c) ΔpilV and d) ΔcomE34 ΔpilV. e) Average fluorescence intensity per cell as a function of time with 3 kbp Cy3-DNA for ΔpilV (black circles), ΔcomE34 ΔpilV (red triangles), ΔcomE1234 ΔpilV (grey squares). Full lines: fits to exponential function. f) Saturation value for average fluorescence intensity per cell obtained from fits in e). g) Characteristic times obtained from fits in e). (N>1500 for each condition).