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Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation

Figure 3

Pre-incubation with DNA inhibits uptake of Cy3-DNA.

a) Probability distribution of the total fluorescence intensity of individual ΔpilV cells after 30 min incubation with Cy3-DNA following incubation with no DNA (black), gDNA (red), 3 kbp fragments (grey). b) Probability distribution of the total fluorescence intensity of individual ΔcomA ΔpilV after 30 min incubation with Cy3-DNA following incubation with no DNA (black), gDNA (red), 3 kbp fragments (grey). c) and d) are the cumulative histograms of a) and b), respectively. (N>1000 for each condition).

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1004043.g003