Substrate-Induced Unfolding of Protein Disulfide Isomerase Displaces the Cholera Toxin A1 Subunit from Its Holotoxin
Figure 1
Loss of PDI structure in the presence of CTA1.
Far-UV CD spectra of PDI (green), CTA1 (blue), and both proteins in the same sample at a 1∶1 molar ratio (red) are shown. The black dotted line was obtained by spectral subtraction of the individual CTA1 and PDI spectra from the spectrum of both proteins together. All measurements were taken at 10°C in pH 7.0 buffer containing 1 mM GSH.