Leishmania-Mediated Inhibition of Iron Export Promotes Parasite Replication in Macrophages
Figure 5
Fpn1 removal from the cell surface stimulates the intracellular growth of L. amazonensis.
BMDM were mock transfected or transfected with Wild type Fpn1-GFP, Fpn1(H32R)-GFP or Fpn1(N144H)-GFP, and after 24 h were treated with hepcidin for 4 h or infected or not with axenic amastigotes of L. amazonensis-RFP for 1 h followed by washes and additional incubation at 34°C for 48 h. The images shown are representative of several live confocal images acquired in 2 independent experiments. The arrows point to Fpn1 on the plasma membrane of BMDM. Bar = 10 µm (applies to all images).