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Red Blood Cell Invasion by Plasmodium vivax: Structural Basis for DBP Engagement of DARC

Figure 6

The structural studies define red blood cell binding.

(A) Adherent HEK293 cells in grey bind to darker, smaller red blood cells when transfected with a DBP-RII surface expression plasmid with a GFP marker. Red blood cell rosetting images for DBP-RII mutants, showing bright field (left), GFP (center), and merged images (right). (B) Percentage of cells expressing point mutants which bind red blood cells, relative to wildtype, shown with standard error. (C) The major DBP-RII∶DARC residues identified in the crystal structures are indicated by red dots. Non-conservative P. knowlesi mutations at critical DBP-RII∶DARC contact residues 274, 356, and 363 suggest why PkDBPα but not PkDBPβ or PkDBPγ bind DARC. (D) Red blood cell rosetting images for DBP-RII receptor specificity mutants, showing bright field (left), GFP (center), and merged images (right). (E) Percentage of cells expressing receptor specificity point mutants which bind red blood cells, relative to wildtype, shown with standard error.

Figure 6

doi: https://doi.org/10.1371/journal.ppat.1003869.g006