The Malarial Serine Protease SUB1 Plays an Essential Role in Parasite Liver Stage Development
Figure 1
PbSUB1 is expressed in exoneme-like subcellular organelles in mature liver stage schizonts.
HepG2 cells infected in vitro with sporozoites of the PbSUB1-HA clone were fixed and examined by IFA, probing with an anti-HA mAb, or anti-GFP antibodies, or antibodies against the PVM protein EXP1. Nuclei were counterstained with 4,6-diamidino-2-phenylindol (DAPI, blue). No HA-specific signal was detected in early schizonts (top row) in which formation of individual merozoites is not yet visible. The images on the second row are shown at higher magnification below in order to better visualize the relative localization of the PbSUB1-specific signal relative to the parasite nuclei and cytosol. Scale bar, 10 µm.