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Serotonergic Chemosensory Neurons Modify the C. elegans Immune Response by Regulating G-Protein Signaling in Epithelial Cells

Figure 3

Increased expression of TPH-1 in ADF chemosensory neurons is caused by reduced contact with contaminated bacterial lawns.

Wild type and egl-30(ad805) animals carrying an integrated tph-1p::DSRED transgene were infected with M. nematophilum or an avirulent form of M. nematophilum using standard (small lawn) or “big lawn” assay conditions. The mean tph-1p::DSRED fluorescence in ADF neurons was quantified. Expression of tph-1p::DSRED was significantly increased when wild type animals were grown on small lawns contaminated with virulent M. nematophilum. This increase in expression was not observed under conditions when animals were unable to leave the bacterial lawn; in egl-30(ad805) animals or when wild type animals were infected on “big lawns”.

Figure 3