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The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies

Figure 2

PGT121–123 variants identified by deep sequencing were used to build phylogenetic trees using ImmuniTree.

(A) An example identity (y-axis) and mutation (x-axis) plot for PGT121 used to identify PGT121-variants for heavy chain and for (B) light chain; each color represents a unique clone. (C) Heavy chain and (D) light chain SHM phylogenies inferred by the ImmuniTree algorithm. Nodes that were inferred by the algorithm are represented as small circles. Nodes representing observed sequences are depicted as larger circles; node size is proportional to the number of reads assigned to that node. The trees are colored based on level of mutation from germline. Previously isolated affinity matured mAbs are labeled (e.g. PGT122) and nodes selected for synthesis and characterization are labeled blue.

Figure 2