Genome-Wide Mouse Mutagenesis Reveals CD45-Mediated T Cell Function as Critical in Protective Immunity to HSV-1
Figure 1
(A) Schematic representation of the breeding strategy used to produce G3 pedigrees. Details of the breeding procedure are described in the Materials and Methods (ENU mutagenesis and breeding section). (B) G3 pedigrees were screened for their susceptibility to HSV-1 infection. Mice were infected i.p. with 1×104 pfu of HSV-1 strain 17 and survival was monitored for two weeks post-infection; susceptible A/J and BALB/c control mice are represented by a blue line, resistant C57BL/6J and C57BL/10J control mice are represented by a green line, all G3 mice by a red line and P43 G3s derived from two G2 daughters (G2a and Gb) and one G1 male by brown and black lines. “n” indicates the number of infected mice for each group.