24 Hours in the Life of HIV-1 in a T Cell Line
RT-qPCR was used to validate key patterns of expression using heat-inactivated virus, primary cells, and natural viral envelope. (A) Analysis of 14 representative genes using competent or heat-inactivated HIV-based vector. The graphs depict the 24 dynamics of expression (log2 fold change of VSV.G pseudotyped HIV-infected over mock) of eight upregulated genes (red lines), five downregulated genes (blue), and one control (RPL31, black line) in SupT1 cells exposed to similar amount of viral particles, only competent HIV (top panel), 1∶10 competent HIV∶heat-inactivated HIV (middle panel), and only heat-inactivated HIV (bottom panel). (B) Analysis in primary CD4+ T cells isolated from two healthy blood donors. Depicted are the 24 dynamics of expression (log2 fold change of VSV.G pseudotyped HIV-infected over mock) of the upregulated (red), downregulated (blue), and control (black) genes. (C) Correlation analysis of RT-qPCR for the 14 representative genes at all time points in primary cells (donor 1) infected by VSV.G or CXCR4 pseudotyped HIV. Log2 fold change linear regression yielded r2 = 0.22, p<10−4.