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24 Hours in the Life of HIV-1 in a T Cell Line

Figure 4

Transcriptome changes upon exposure to infectious and non-infectious viral particles.

Principal component analysis is used to explore the overall variance structure of the transcriptome datasets. With each point representing a whole transcriptome sample, the figure presents the transcriptome of cells that were universally infected (HIV), cells exposed to heat-inactivated virus (Heat-inactivated), cells exposed to a mixture of 1∶10 infectious/heat-inactivated virus (HIV[1/10]), and non-infected cells (Mock). One mock sample failed and is not plotted. The transcriptome of mock cells and that of cells exposed to heat-inactivated viruses clustered together across the top principal components. Infected cells, on the other hand, spread away from the mock space as infection progressed, with the most distant dot corresponding to the latest time point (24 h). The mixture 1/10 infectious/noninfectious material occupies the intermediate space. Clustering of the two hours samples corresponds to end of cell exposure to the virus or control materials.

Figure 4