Assembly of the Type II Secretion System such as Found in Vibrio cholerae Depends on the Novel Pilotin AspS
Phylogenetic tree reconstruction was performed with PhyML v3.0  using 500 bootstrap calculations and shown as percentage values (for further details see Methods). Based on the strong statistical support in the division, the Vibrio-type and Klebsiella type secretin sub-families are highlighted with colour. These have also been labelled as “GspDα” and “GspDβ” in accordance with a new nomenclature recently proposed for ETEC str. H10407 . The branch to the secretin SttD in Dickeya spp. is not shown to full scale. For a full list of the sequence accession numbers see Table S2.