Hsp90 Inhibitors Are Efficacious against Kaposi Sarcoma by Enhancing the Degradation of the Essential Viral Gene LANA, of the Viral Co-Receptor EphA2 as well as Other Client Proteins
Figure 6
Effects of Hsp90 inhibitors on LANA in PEL cells.
(A–D) Hsp90 inhibitors repress LANA expression in PEL cells. PEL cells including BC-3, BCP-1, BCBL-1, and BC-1 cells were treated with 17-DMAG at concentrations of 0, 0.1, 0.5, 2.5 and 10 µM and after 48 hours, whole cells lysates were immunoblotted with anti-LANA, anti-Hsp90, anti-Akt, and anti-cleaved caspase-3 antibodies, Cdc2 and β-Actin were used as controls. (E–G) Apoptosis. PEL cells including BC-3, BCP-1, BCBL-1, and BC-1 cells were treated respectively with Hsp90 inhibitors 17-DMAG and PU-H71 at concentrations of 0, 1, and 10 µM, and 10 µM, or 0, 0.1 µM AUY922 for 24 hours, apoptosis percentage was analyzed after PEL cells were harvested and stained.