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A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa

Figure 6

Detection of antibodies to BASV by serum neutralization of VSVΔG-GFP pseudotypes.

Infectivities of VSVΔGFP pseudotypes bearing the glycoproteins of VSV or BASV, respectively, after incubation with 5-fold serial dilutions (1∶10, 1∶50, 1∶250, 1∶1,250, 1∶6,250, 1∶31,250) of sera from six individuals are depicted as percent of infectivity in the absence of serum. The six individuals tested include a patient with hemorrhagic fever (panel A, “Patient 3”), the nurse directly caring for him (panel F, “Contact 5”), and other health care workers in Mangala village (panels B–E). All data points represent the average of triplicate assays; error bars indicate standard deviations. Similar results were obtained in an independent experiment using murine leukemia virus (MLV)-based pseudotypes (data not shown).

Figure 6