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A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa

Figure 2

Deep sequencing and whole-genome de novo assembly of BASV.

After initial discovery of BASV from a single 454 pyrosequencing read, 98.2% of the BASV genome was assembled de novo from >140 million paired-end Illumina reads. The horizontal lines (red) depict regions of the genome successfully assembled at the end of each cycle. PCR and Sanger sequencing were performed to confirm the assembly and genomic organization of BASV (green lines).

Figure 2