Germline Transgenesis and Insertional Mutagenesis in Schistosoma mansoni Mediated by Murine Leukemia Virus
Figure 3
Integration events within the genome of MLV-transduced Schistosoma mansoni.
Panel A: Horizontal bars represent the chromosomes of S. mansoni. MLV integration sites shown above the chromosomes were detected in genomic DNA from transduced adults and schistosomules (somatic transgenesis approach). MLV integration sites shown below the chromosomes were detected in genomic DNA from cercariae derived from MLV-transduced eggs/miracidia (germline transgenesis approach). Colors of integration sites follow the color code indicated in panel B. The scale bar indicates physical distances of the map. Panel B: Left, pie chart showing the percentages of the indicated regions in the genome of S. mansoni. Right, pie chart showing the percentages of MLV integration sites classified according to the function of the mapping site; Panel C: Box plots showing expression levels of genes carrying MLV transgenes (green) and transcriptomes at large (yellow) of the indicated developmental stages. A1, wild-type adults; A2, transgenic adults; C1, wild-type cercariae; C2, transgenic cercariae; S1, wild-type schistosomules; S2, transgenic schistosomules. RPKM, reads per kilobase per million mapped reads.