Large-Scale Gene Disruption in Magnaporthe oryzae Identifies MC69, a Secreted Protein Required for Infection by Monocot and Dicot Fungal Pathogens
(A) MC69 is required for pathogenicity of M. oryzae strain 70-15. Conidial suspension of the wild-type strain 70-15 (70-15 WT) and the mc69 mutants (mc69-119, -31) were inoculated on barley (cv. Nigrate) and rice (cv. Shin No. 2) leaves, and incubated for 7 days. (B) Germination, appressorium formation and appressorial penetration of 70-15 WT and the mc69 mutants. The ratio of germination was calculated as the mean percentage of conidia germinated after 32 h on rice (cv. Shin No. 2) leaf sheath cells. The mean percentage of appressorium formation on rice leaf sheath cells among the germinated conidia is presented. Three replicates of ∼50 conidia were counted for each observation. The mean percentage of appressorial penetration by the mc69 mutants is presented 32 h after inoculation. Standard errors are indicated by the vertical bars.