Large-Scale Gene Disruption in Magnaporthe oryzae Identifies MC69, a Secreted Protein Required for Infection by Monocot and Dicot Fungal Pathogens
Merged DIC and mCherry images of rice leaf sheath cells infected by M. oryzae Sasa2 strain harboring (A) PWL2p::PWL2::mCherry::NLS, (B) PWL2p::MC69::mCherry::NLS, and (C) PWL2p::MC69::mCherry 24, 27 and 32 h after inoculation as observed by confocal laser scanning microscopy. Arrows indicate BICs and triangles indicate rice nuclei. Pinhole setting is 240 µm for all panels. Scale bar = 20 µm.