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Large-Scale Gene Disruption in Magnaporthe oryzae Identifies MC69, a Secreted Protein Required for Infection by Monocot and Dicot Fungal Pathogens

Figure 3

MC69::mCherry is not translocated into the rice cytoplasm.

Merged DIC and mCherry images of rice leaf sheath cells infected by M. oryzae Sasa2 strain harboring (A) PWL2p::PWL2::mCherry::NLS, (B) PWL2p::MC69::mCherry::NLS, and (C) PWL2p::MC69::mCherry 24, 27 and 32 h after inoculation as observed by confocal laser scanning microscopy. Arrows indicate BICs and triangles indicate rice nuclei. Pinhole setting is 240 µm for all panels. Scale bar = 20 µm.

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1002711.g003