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Synchronized Retrovirus Fusion in Cells Expressing Alternative Receptor Isoforms Releases the Viral Core into Distinct Sub-cellular Compartments

Figure 4

NH4Cl arrest creates large endosomes.

(A) TVA950 cells co-expressing CFP-Rab5 (green) and YFP-Rab7 (red) before (top row) and after (bottom row) incubation with 70 mM NH4Cl for 40 minutes at 37°C. Enlarged endosomes positive for Rab5 (diameter 12 µm) and Rab7 (diameter 15 µm) are indicated by arrowheads and arrows, respectively. Scale bars are 20 µm. (B) Same as in panel A, but for TVA800 cells. Enlarged endosomes positive for Rab5 (diameter 3.5 µm) and Rab7 (diameter 5 µm) are indicated by arrowheads and arrows, respectively. Scale bar 15 µm.

Figure 4

doi: https://doi.org/10.1371/journal.ppat.1002694.g004