Structural Basis of Cytotoxicity Mediated by the Type III Secretion Toxin ExoU from Pseudomonas aeruginosa
Figure 5
Ub-ExoU is targeted to endosomes.
(A) Co-localization of Ub-ExoU with endosomal markers. ExoU S142A-VC was co-transfected with VN-Ub (upper panel) or with VN-Ub K63 (bottom panel). 36 h later, cells were stained with the anti-EEA1, or treated with lysotracker red. Pearson's correlation coefficients correspond to 0.692, 0.570, and 0.801 the top left, top right, and bottom panels, respectively. (B). HeLa cells were infected for 3 h with PaΔST+ExoU S142A (upper panel), PaΔST+ExoU S142A-K178R or PaΔST+ExoU S142A Δ679–683 (bottom panel), fixed and immunolabeled with anti-ExoU and anti-EEA1 antibodies. Single two-channel confocal images are presented. The Pearson's correlation coefficient for EEA1-ExoU=0.213. Bars, 12 µm.