Molecular Determinants of Resistance Activation and Suppression by Phytophthora infestans Effector IPI-O
A) Yeast two-hybrid interactions between domains of RB or domain self-association; B) Interactions between domains of RB and IPI-O1 or IPI-O4; C) Results of co-immunoprecipitation of RB CC domain with IPI-O fusion proteins. The indicated protein combinations were expressed in N. benthamiana leaves and total proteins were incubated with green fluorescent protein (GFP) antibody and agarose beads. Precipitated proteins were detected using Myc-tag antibody. Proteins with no fusion or with a hemagglutinin (HA) tag were used as negative controls.