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Structural Insights into a Unique Legionella pneumophila Effector LidA Recognizing Both GDP and GTP Bound Rab1 in Their Active State

Figure 6

13 kinds of Rab GTPase family members could be recognized by LidA in vitro.

(A) Sequence alignment of fifteen different Rabs. The LidA-interacting residues in Rab1 are depicted by triangles. The two sequences underneath the black dashed lines correspond to the members unbound to LidA. (B) The GST-pull down of LidA(FL) by beads-immobilized 15 kinds of GST-Rab family members, Rab20 was His-tagged for exceptional.

Figure 6

doi: https://doi.org/10.1371/journal.ppat.1002528.g006