Concerted Actions of a Thermo-labile Regulator and a Unique Intergenic RNA Thermosensor Control Yersinia Virulence
Figure 7
Temperature-dependent binding of ribosomes to the yscW-lcrF intergenic region.
Toeprinting analysis was performed with the wildtype, the repressed (UU-28/-27CC) and derepressed (GUU-30/-28AAA) variants as described in material and methods. The presence (+) and absence (−) of the 30S ribosomal subunits are indicated. The terminated primer extension products (toeprints) are marked. The sequencing ladder (ACGU) generated with the same lcrF-specific primer is loaded on the left. The positions of the fourU motif, the Shine-Dalgarno sequence and the start codon AUG are indicated.