Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma
Figure 3
Identification of dcr-2 null mutant mosquito cell lines.
Size distribution and nucleotide analysis of virus-derived small RNAs in u4.4 cells (A), dcr-2FS−1 (C6/36) cells (B) and dcr-2del 33 (C7-10) cells (C) infected with CHIKV. Schematics indicating Dcr-2 domains (D). The A. albopictus Dcr-2 contains a DExH/D protein family domain (DEAD) and helicase conserved C-terminal domain (H); a domain of unknown function (DUF); a PAZ domain; and tandem RNase III domains. Asterisks indicate locations of deletions in dcr-2 sequences.