The Human Cytomegalovirus UL11 Protein Interacts with the Receptor Tyrosine Phosphatase CD45, Resulting in Functional Paralysis of T Cells
Figure 5
Surface expressed pUL11 mediates cell adhesion.
(A) PBMCs, Jurkat or J-AS-1 cells were incubated with human fibroblasts that were transduced 3 days earlier with recombinant adenoviruses expressing pUL11 and GFP (rAdV UL11) or GFP alone (rAdV GFP). Unbound cells were removed by washing. White arrows indicate adhering cells, green cells show adenovirus derived GFP. Size bar, 50 µm. (B) Human fibroblasts transduced and depicted as in (A) were left untreated (top row), or incubated for 2 h with anti-UL11 (middle row) or pre-immune rabbit serum (bottom row) prior to incubation with Jurkat cells. Unbound cells were removed by washing. Size bar, 50 µm.