Sialidases Affect the Host Cell Adherence and Epsilon Toxin-Induced Cytotoxicity of Clostridium perfringens Type D Strain CN3718
Figure 13
Attachment of C. perfringens to Caco-2 cells.
Caco-2 cell monolayers were incubated for 2 h with bacteria at 37°C under anaerobic conditions. Monolayers were then washed three times with HBSS buffer, lysed in distilled water and total bacteria were plated onto BHI agar plates for counting. Panel A, Attachment levels expressed as the percentage of attached bacteria relative to the total number of input bacteria. The results shown represent the average of three repetitions; the error bars indicate standard errors of the means. Panel B, Attachment levels expressed as the bacteria attached per mammalian cell. The results shown represent the average of three repetitions; the error bars indicate standard errors of the means. Panel C, C. perfringens BMC206, BMC207 and BMC2072 attached to Caco-2 cells as detected by fluorescence microscopy (600×) as described in the Materials and Methods. Green: bacteria; Red: Caco-2 cells.