Evolution of Multidrug Resistance during Staphylococcus aureus Infection Involves Mutation of the Essential Two Component Regulator WalKR
Virulence analysis using the Galleria mellonella model demonstrating percent survival over 6 days using a Kaplan Meier plot. The parent VSSA strain JKD6009, the clinically derived VISA JKD6008, and the laboratory derived mutant TPS3130 are included. Included also for comparison is the virulent community clone of methicillin-resistant S. aureus, USA300. The difference in survival between JKD6009 and JKD6008 or TPS3130 was statistically significant (p<0.0001).